Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. This format is available for the low molecular weight, 50 bp, 100 bp and 1 kb plus dna ladders. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose gel. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. The doublestranded dna is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 rnm trishci ph 8. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Invitrogen trackit dna ladders are designed for use with agarose gels and are provided in convenient, readytoload formats. All rna molecules in the ladder are polyatailed 40 nt for convenient use as control templates for cdna synthesis.
Invitrogen trackit 1 kb plus dna ladder 100 applications nucleic acid molecular weight markers biochemicals and reagents. All include a vial of gel loading dye, purple 6x, no sds. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. To create our dna ladders, each fragment is purified individually using proprietary chromatographybased technology, resulting in dna ladders of superior quality which exceeds industry standards.
In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. The invitrogen 1kb plus dna ladder is designed for. Amount of dna ladder per lane was loaded according to each manufacturers recommendation. Compare rna ladders from leading suppliers on biocompare. View more information about our dna stains technology. The invitrogen 1 kb plus dna ladder thermo fisher scientific. The ladder is prepared from a plasmid containing repeats of a 1018 bp dna fragment. The 1 kb and 3 kb bands contain more dna to provide internal orientation. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. Invitrogen 1kb dna extension ladder 100ug nucleic acid. Alternatively, the dna ladder can be diluted in a buffer such that the final concentration of nacl is 20 mm.
The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and. By the use of these products you accept the terms and conditions of all applicable limited use label licenses. Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need invitrogen. Invitrogen recommends the use of 10x bluejuice gel loading buffer 10816015 at a concentration of 2x for electrophoresis of this ladder on agarose gels. The 100 bp dna ladder consists of 15 bluntended fragments between 100 and 1500 bp in multiples of 100 bp and an additional fragment at 2072 bp.
The product is supplied in a solution of 10 mm trishcl, ph 8. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. Nebs quickload purple dna ladders offer sharp, uniform bands with easily identifiable reference bands. Invitrogen trackit 1 kb plus dna ladder 100 applications. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. Trackit dna ladders are supplied premixed in loading buffer containing tracking dyes that run behind and ahead of the sample, ensuring the dna bands will not be obscured by the dyes.
Trackit dna ladders are stable at room temperature and can be stored on your benchtop. Do not heat the trackit 1kb plus dna ladder before loading. If samples need to be diluted, use te or other buffer of minimal ionic strength. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick. The 1636 bp and all bands 1 kb dna ladder is formulated with unique tracking. This product is available in quickload purple format enjoy the convenience of a readytoload format. Invitrogen dna ladders are provided with the loading buffer in a separate tube.
This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. How can i prepare working solution with 1 kb dna ladder. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. The invitrogen trackit 1kb plus dna ladder is designed for. Egel 1 kb plus dna ladder thermo fisher scientific. One kb plus dna ladder is compatible with agarose gels cast in lab as well as egel precast agarose gels. The dna ladder consists of 14 dna fragments and is provided with 6x. Please follow the gel staining protocol provided by the manufacturer of. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple 6x, no sds.
The ladder consists of nine bands in even increments 0. Do not heat the 1kb plus dna ladder before loading. One kb plus dna ladder is most popular dna ladder used to estimate molecular mass of doublestranded dna bands from pcr, restriction digests, and other molecular biology protocols. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder.
New england biolabs uk ltd quickload 1 kb dna ladder. See links for brands thermo scientific applied biosystems invitrogen. The ladder is composed of fourteen chromatographypurified individual. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Invitrogen mass dna ladders are designed for accurate quantification and sizing of dna fragments in agarose gels. The bands of the ladder each contain from 1 to 12 repeats of a 1018. The trackit 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 500 bp12 kb. Invitrogen dna ladders are available for a wide variety of size ranges, applications, and formats. The key to accurate band analysis is to use the correct dna ladder for your particular application.
Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Generuler 1 kb dna ladder thermo fisher scientific. Use the 1 kb plus dna ladder is to size linear doublestranded dna fragments from 100 bp to 12 kb. There is no need to heat, mix, or dilute these ladders prior to loading them on your gel. There have been no changes to product formulation or specifications. Invitrogen egel 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. For optimal resolution, the recommended agarose gel concentration is 0. Not intended for any animal or human therapeutic or diagnostic use.
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